Identificaciones de ssquazzo

Voucher collection 10522dll was collected on 13 November 2021 from the McKay tract forest east of Winship Middle School in Cutten, Humboldt County, California. The collection consisted of 3 fruiting bodies scattered in redwood branchlet humus on the edge forest dominated by Coast Redwood and Douglas fir. Microscopic and Macrosopic Features were described, Photos taken by Pamela Largent, and an ITS sequence was provided by Sharon Squazzo.

Description of the Microscopic Features of 10522dll

Basidiospores 5–6 angled, angle distinct, spore apex acute or obtuse, most spores with a single droplet, isodiametric to heterodiametric in profile view, on the average isodiametric, 7.0–9.5 × 5.0–7.0 μm (xavg = 8.3 × 6.14 μm 8.1 × 7.3 ± 0.47 μm; Q = 1.17–1.61; Qavg = 1.35 ± 0.10; n = 49).
Basidia clavate and tapering to a narrow base or cylindro-clavate and narrowing to a moderately broad base, not separable when crushed, 29.–44.5 × 8.–10.4 μm (xavg = 36.6 × 9.4 μm; Q = 3.2–4.94; Qavg =3.2 ± 0.55; n = 54); 4-sterigmate, often thin and thread-like.
Cheilocystidia observed only from gill sections were scattered to abundant , cylindrical, colorless, 23.0–103.0 × 3.0–19.5 μm (xavg = 50.8 × 8.9 μm; Q = 3.0–9.9; Qavg = 6.16 and; n= 17).
In mature specimens the Pileipellis up to ~220 μm deep and a Palisadoderm on the pileus disc, composed of numerous erect, tightly entangled hyphae with the terminal 2 to 3 cells inflated and with a greyish blue intracellular pigment that has a distinct brownish cast in 3% KOH; versiform in shape (cylindrical, clavate, fusiform, napiform, and vesiculate), 18.0–141.5 × 6.0–75.0 μm (xavg = 70.3 × 19.6 μm; Q = 1.3–12.5; Qavg = 4.08; n = 56)
Stiptipellis longitudinal stipe sections at the stipe apex composed of abundant, erect to at time semi-erect, colorless caulocystidia in between which are a layer of 3–4 prostrate colorless hyphae, everywhere else the clusters of caulocystidia are absent; Caulocystidia cylindrical and therefore resemble the cheilocystidia in shape, measure 9.0–45.5 × 2.0–9.5 greyish blue intracellular pigment that has a distinct brownish cast in 3% KOH (xavg = 26.8 × 5.4 μm; Q = 3.2–11.4; Qavg = 5.06; n = 16).
Clamp connections absent in all tissue.
Pigment in Pileipellis and Pileocystidia have a greyish blue intracellular pigment that has a distinct brownish cast in 3% KOH, Stipitipellis colorless in 3% KOH, encrusted on the outer walls of the hyphae in the pileus trama, the encrustations in the pileus are distinct, those in the stipe trama are indistinct. The pigment in the gill trama was not recorded.
Oleiferous Hyphae abundant in the pileus, lamellar, and stipe tramas.
Lipoidal Globules in Pileipellis and above in 3 % KOH.
Stipe Tramal hyphae without granules.

Comments: The species has broadly clavate bluish grey pileus that becomes greyish with maturity; a tomentulose pileus and minutely squamulose elsewhere which when mature the squamules are distinct from grey pileus. The pileus margin becomes wavy and lobed with maturity. The pileus margin is opaque (= not translucent-striate) at all times. The odor and taste are mild. The distinct microscopic features: length of the 5-6 angled basidiospores is less than 10 μm, it has cylindrical, colorless cheilocystidia and caulocystidia, a palisadoderm pileipellis, versiform pileocystidia, and abundant granules. It my 1994, this collection keys out to Leptonia foliocontusa var. discolor. In 2009, this species was treated as synonym of Entoloma foliocontusum (Largent) Noordel. & Co-David. Unfortunately, the variety was not treated as a synonym in the 2009 publication

HAY-F-004189
Grand Point, Somerset, CA. 2800' elevation between summits on the north face.
Burnt standing conifer with blackberry and poison oak at the edge of a grassland.
Substrate- Wood/burnt wood.
KOH- slight darkening.
UV- pink on mycelium with some blueing. A brightening on the cap surface.
Odor- Sweet pine, sugary and woody.
Taste-N/A
Pore opening with beetles inside. Cap yellowish pale with some speckling.

-One mile upriver at Purdon crossing south Yuba River. NE bank 10’ from river.

• Madrone, Black oak and interior live oak.
  -Growing on oak branches and trunk covered in black oak leaf litter and twigs.
  -KOH became greenish.
  -No UV

• Fruiting with decayed false turkey tail, T. Betulina and an ant colony.

HAY-F-004183
Ingahee Rd, Pollock Pines. 100’ from a dam on the valley floor NE bank, 10’ from water's edge.
Cedar, pine, fir, alder and maples. Blackberry abundant.
Growing on a standing dead needle-bearing tree.
Hardwood substrate.
No odor. No taste.
KOH negative/indistinct.
UV negative.
Flesh firm but fluffy. Growing out from under scales of bark. Like bubbly burnt charcoal. One other tree affected within 5’. When bitten into flesh was ‘feathery soft’